golden gate assembly kit (New England Biolabs)
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New England Biolabs
golden gate assembly kit
Golden Gate Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/golden+gate+assembly+kit/NEBridge+Golden+Gate+Assembly+Kit/pm42754924-51-28-27
Average 99 stars, based on 324 article reviews
Golden Gate Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/golden+gate+assembly+kit/NEBridge+Golden+Gate+Assembly+Kit/pm42754924-51-28-27
Average 99 stars, based on 324 article reviews
golden gate assembly kit - by Bioz Stars,
2026-10
99/100 stars
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Amplification:Article Title: β-Carbonic anhydrases integrate CO 2 and light cues to coordinate photosynthesis in tomato. Article Snippet: Optimization of photosynthesis requires precise coordination between electron transport and CO2 assimilation, but how plants maintain this coordination under suboptimal CO2 and light conditions remains poorly understood.. Here, we identify two tomato (Solanum lycopersicum) b-carbonic anhydrases (bCAs) responsive to deficient CO2.. Although both are required for biomass accumulation and efficient photosynthesis, the chloroplast bCA1 does not directly participate in carbon assimilation but instead specifically coordinates photosystem performance and leaf internal structure. Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISPRn library was cloned into lentiGuide-Puro (Addgene, cat. no. 52963) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, cat. no. E2621L) according to manufacturer specifications. .. The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Clone Assay:Article Title: β-Carbonic anhydrases integrate CO 2 and light cues to coordinate photosynthesis in tomato. Article Snippet: Optimization of photosynthesis requires precise coordination between electron transport and CO2 assimilation, but how plants maintain this coordination under suboptimal CO2 and light conditions remains poorly understood.. Here, we identify two tomato (Solanum lycopersicum) b-carbonic anhydrases (bCAs) responsive to deficient CO2.. Although both are required for biomass accumulation and efficient photosynthesis, the chloroplast bCA1 does not directly participate in carbon assimilation but instead specifically coordinates photosystem performance and leaf internal structure. Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISPRn library was cloned into lentiGuide-Puro (Addgene, cat. no. 52963) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, cat. no. E2621L) according to manufacturer specifications. .. The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs, cat. no. E1602L) following the manufacturer’s protocol. .. Individual sgRNAs for arrayed CRISPRa validations were constructed by Golden Gate Cloning as described in.86 Briefly, DNA oligomers with Golden Gate overhangs were annealed and cloned into a non-digested target plasmid, pXPR_502, using the NEB Plasmid Preparation:Article Title: β-Carbonic anhydrases integrate CO 2 and light cues to coordinate photosynthesis in tomato. Article Snippet: Optimization of photosynthesis requires precise coordination between electron transport and CO2 assimilation, but how plants maintain this coordination under suboptimal CO2 and light conditions remains poorly understood.. Here, we identify two tomato (Solanum lycopersicum) b-carbonic anhydrases (bCAs) responsive to deficient CO2.. Although both are required for biomass accumulation and efficient photosynthesis, the chloroplast bCA1 does not directly participate in carbon assimilation but instead specifically coordinates photosystem performance and leaf internal structure. Article Title: Parallel CLE peptide signaling pathways control nodulation in pea Article Snippet: .. PsCLE12 and PsCLE13 under the control of a CAMV35S promoter were inserted in the pCAMBIA_CR1 (pCR1) vector that contains the DsRED transformation marker (Sevin-Pujol et al., 2017) using the Article Title: Techno-economic assessment-guided biofoundry for microbial strain development. Article Snippet: Yu Been Heo, Sung Cheon Ko, Jay D. Keasling, Han Min Woo hmwoo@skku.edu (H.M.. Woo).. Trends in Biotechnology, Month 2025, Vol. xx, No. xx https://doi.org/10.1016/j.tibtech.2025.11.002 TIBTEC 2849 No. of Pages 24 Trends in Biotechnology Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs, cat. no. E1602L) following the manufacturer’s protocol. .. Individual sgRNAs for arrayed CRISPRa validations were constructed by Golden Gate Cloning as described in.86 Briefly, DNA oligomers with Golden Gate overhangs were annealed and cloned into a non-digested target plasmid, pXPR_502, using the NEB Polymerase Chain Reaction:Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISPRn library was cloned into lentiGuide-Puro (Addgene, cat. no. 52963) using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, cat. no. E2621L) according to manufacturer specifications. .. The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Control:Article Title: Parallel CLE peptide signaling pathways control nodulation in pea Article Snippet: .. PsCLE12 and PsCLE13 under the control of a CAMV35S promoter were inserted in the pCAMBIA_CR1 (pCR1) vector that contains the DsRED transformation marker (Sevin-Pujol et al., 2017) using the Transformation Assay:Article Title: Parallel CLE peptide signaling pathways control nodulation in pea Article Snippet: .. PsCLE12 and PsCLE13 under the control of a CAMV35S promoter were inserted in the pCAMBIA_CR1 (pCR1) vector that contains the DsRED transformation marker (Sevin-Pujol et al., 2017) using the Marker:Article Title: Parallel CLE peptide signaling pathways control nodulation in pea Article Snippet: .. PsCLE12 and PsCLE13 under the control of a CAMV35S promoter were inserted in the pCAMBIA_CR1 (pCR1) vector that contains the DsRED transformation marker (Sevin-Pujol et al., 2017) using the Construct:Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs, cat. no. E1602L) following the manufacturer’s protocol. .. Individual sgRNAs for arrayed CRISPRa validations were constructed by Golden Gate Cloning as described in.86 Briefly, DNA oligomers with Golden Gate overhangs were annealed and cloned into a non-digested target plasmid, pXPR_502, using the NEB Article Title: Assessment of the phi6 lysis system using genetic complementation and heterologous expression in Escherichia coli and Pseudomonas syringae Article Snippet: .. The pJLC140 shuttle vector was constructed using the Cloning:Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: The secondary CRISRPa library was amplified by PCR (15 cycles) with golden gate compatible primers and cloned into pXPR_502 (Addgene, cat. no. 96923) using NEB Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs, cat. no. E1602L) following the manufacturer’s protocol. .. Individual sgRNAs for arrayed CRISPRa validations were constructed by Golden Gate Cloning as described in.86 Briefly, DNA oligomers with Golden Gate overhangs were annealed and cloned into a non-digested target plasmid, pXPR_502, using the NEB Concentration Assay:Article Title: Systematic genome-wide mapping of host determinants of bacteriophage infectivity Article Snippet: PCR was performed using KAPA HiFi (Roche KK2101) for all experiments, except for colony PCR, where KAPA2G Robust (Roche KK5005) was utilized. .. The New England Biosciences (NEB) |